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雙鏈聚肌胞 |
| CAS No.: |
42424-50-0 |
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中文名稱雙鏈聚肌胞中文同義詞聚肌苷酸-聚胞苷酸鈉鹽;雙鏈聚肌胞;6.雙鏈聚肌胞(POLYI:C.);卡那霉素-聚肌胞鈉鹽復合物;雙鏈聚肌胞鈉鹽;雙鏈聚肌胞溶液;POLYI:C雙鏈聚肌胞鈉鹽;聚肌苷-聚胞苷酸鈉鹽英文名稱Polyinosinic-polycytidylicacidsodiumsalt英文同義詞POLYINOSINICACID+POLYCYTIDYLICACID,SODIUMSALT;Polymyocytesodiumsalt;POLYINOSINICACIDPOLYCYTIDYLICACID,SODIUMSALT;POLYINOSINIC-POLYCYTIDYLICACIDSODIUMSALT;POLYIPOLYC,NA;POLY[I]POLY[C]SODIUMSALT;Oligochitosan-kanamycin-polyI-polyCsodiumsaltcompoundsolution;Polyinosinicacid-polycytidylicacidsodiumsalt,double-strandedCAS號42424-50-0分子式C19H27N7O16P2分子量671.41EINECS號1308068-626-2相關類別醫(yī)藥中間體;中間體;獸用鉰料添加劑和嬰幼兒奶粉添加;醫(yī)藥獸藥;原料藥;生化試劑-聚合物類;醫(yī)藥原料;化學試劑;BiochemicalsandReagents;Biomaterials;CellCulture;MaterialsScience;Nucleosides;Nucleotides;OligonucleotidePolymersandCopolymers;Oligonucleotides;Otheradjuvants;SyntheticOligonucleotidesandRelatedReagents;VaccineAdjuvants;VaccineProductionMol文件42424-50-0.mol結構式雙鏈聚肌胞性質儲存條件?20°C溶解度H2O：可溶，10mg/mL形態(tài)凍干粉顏色白色至米白色水溶解性Water:10mg/mL(Needultrasonicandwarming)雙鏈聚肌胞用途與合成方法生物活性Polyinosinic-polycytidylicacidsodium(Poly(I:C)sodium)是雙鏈RNA的合成類似物，是一種TLR3和視黃酸誘導型基因I受體(RIG-I和MDA5)的激動劑。Polyinosinic-polycytidylicacidsodium可以用作疫苗佐劑，以增強先天性和適應性免疫反應，并改變腫瘤的微環(huán)境，還可以直接觸發(fā)癌細胞發(fā)生凋亡。靶點TLR3,RIG-I,MDA5,apoptosis體外研究Polyinosinic-polycytidylicacid(20ng/mL;24hours;WM793,WM278,WM239A,WM9and1205Lucells)treatmentstronglyreducesviabilityfrom100%incontrolsto20%–50%within24hours.Polyinosinic-polycytidylicacid(200ng/mL;24hours;1205Lucells)treatmentinducesapoptosisin1205Lucells.Polyinosinic-polycytChemicalbookidylicacid(3ng/mL;24hours;1205Lucells)treatmentinducesIFN-βexpressioninmelanomacells.SilencingofRIG-IandMDA-5confirmedthatinductionofIFN-βbyPolyinosinic-polycytidylicacidrequiredRIG-IandMDA-5,respectively,andthatrequiredIPS-1.Polyinosinic-polycytidylicacid(5ng/mL;24hours;1205Lucells)treatmentrevealsactivesubunitsofcaspase-9andcaspase-8inmelanomacells.Polyinosinic-polycytidylicacidsodiumispreparedforinjectionbyresuspendinginsterilesaline,heatingto50°Cataconcentrationof2mg/mltoensurecompletesolubilisationandthenallowingtocoolnaturallytoroomtemperaturetoensureproperannealingofdouble-strandedRNA.PolyI:Cisstoredat-20°Cuntiluse.CellViabilityAssayCellLine:WM793,WM278,WM239A,WM9and1205LucellsConcentration:20ng/mLIncubationTime:24hoursResult:Stronglyreducedviabilityfrom100%incontrolsto20%–50%within24hours.ApoptosisAnalysisCellLine:1205LucellsConcentration:200ng/mLIncubationTime:24hoursResult:Inducedapoptosisin1205Lucells.RT-PCRCellLine:1205LucellsConcentration:3ng/mLIncubationTime:24hoursResult:InducedIFN-βexpressioninmelanomacells.WesternBlotAnalysisCellLine:1205LucellsConcentration:5ng/mLIncubationTime:24hoursResult:Revealedactivesubunitsofcaspase-9andcaspase-8inmelanomacells.體內研究Polyinosinic-polycytidylicacidtreatmentinhibitstumorgrowthinNOD/SCIDimmunodeficientmiceinjectedwith1205Lucells.ThelevelofhumanDNAis50%lowerinmicetreatedwithPolyinosinic-polycytidylicacid.用途一種可作為在TLR3水平對細胞信號轉導進行研究的模型RNA的雙鏈均聚物。也是一種TRIF依賴性toll樣受體-3(TLR3)配體。 |
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