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熒光素 |
| CAS No.: |
2321-07-5 |
| 分子式： |
C20H12O5 |
| 分子量： |
332.31 |
| 備注： |
中文名稱熒光素中文同義詞熒光橙紅;熒光素,指示劑;黃7[CI45350:1];熒光素,水溶;熒光酯;螢光橙黃;螢光紅;螢光黃英文名稱Fluorescein英文同義詞FLUORESCEIN,FREEACIDFLUORESCEIN,FREEACIDFLUORESCEIN,FREEACID;Diresorcinolphthalein;Tetraoxyphthalophenoneanhydride;SOLVENTYELLOW94;SNO880;RESORCINOLPHTHALEIN;FLUORESCEIN,ALCOHOLSOLUBLE;FLUORESCEINCAS號(hào)2321-07-5分子式C20H12O5分子量332.31EINECS號(hào)219-031-8相關(guān)類別滴定;分析試劑;標(biāo)準(zhǔn)品;色素類;生化試劑;生物染色劑;小分子抑制劑;基因組學(xué)和分子診斷;化合物;生化試劑-色素類;化學(xué)試劑;化工原料;大化工;E-FFluorescentProbes,Labels,ParticlesandStains;FluoresceinandDerivatives;FluorescentLabels;StainsandDyes;Stains&Dyes,Ato;Aromatics;FluorescentLabels&Indicators;Heterocycles;Intermediates&FineChemicals;Pharmaceuticals;FUNDUSCEIN;Fluorescent;Xanthene;化工材料;有機(jī)試劑;通用生化試劑-生物染色劑與化學(xué)指示劑;染料;試劑盒;日化品原料;有機(jī)化工原料Mol文件2321-07-5.mol結(jié)構(gòu)式熒光素性質(zhì)熔點(diǎn)320°C(lit.)沸點(diǎn)429.44°C(roughestimate)密度1.2739(roughestimate)折射率1.5000(estimate)儲(chǔ)存條件roomtemp溶解度不溶于水、乙醚、苯、氯仿；溶于乙醇、甲醇、丙酮、乙酸乙酯、N,N-二甲基甲酰胺酸度系數(shù)(pKa)2.2,4.4,6.7(at25℃)色指數(shù)45350形態(tài)粉末顏色紅色到橙色酸堿指示劑變色ph值范圍Pinkuorescence(4.0)togreenuorescence(6.0)水溶解性insoluble最大波長(zhǎng)(λmax)493.5nm,496nm,460nm,515nmMerck14,4159BRN94324穩(wěn)定性穩(wěn)定的。易燃。與強(qiáng)氧化劑不相容。主要應(yīng)用Organiclight-emittingdiode,nanoparticles,liquidcrystaldisplay,oilproducts,lipmake-up,nucleicacidsynthesis,amplification,sequencingandcloning,diagnosisofdiabeticretinopathy,detectingyeast,multidrugresistance,proteins,antiHCVantibodies,targetgenes,enzymaticactivity,nerveagent,nucleicacidsequences,imaginglungcancer,prostatecancer生物領(lǐng)域應(yīng)用Detectingchromosomalaberration;diagnosingcornealdiseases;marking/detectinginsects;asantitumoragent;asantihistaminicagent;useinagricultureandplantcultivation;useincosmetics;useinophthalmology;asasubstrateformeasuringα-amylasesactivity,elastasesactivity,esterasesactivity,βChemicalbook-glucuronidasesactivity,horseradishperoxidasesactivity,kinasesactivity,monoamineoxidases(MAOs)activity,phosphatasesactivity,phospholipasesactivity,proteases/proteinasesactivity,telomerasesactivityactivityLogP2.980(est)CAS數(shù)據(jù)庫(kù)2321-07-5(CASDataBaseReference)EPA化學(xué)物質(zhì)信息Fluorescein(2321-07-5)熒光素用途與合成方法生物活性Fluorescein(Resorcinolphthalein)是一種合成的有機(jī)光敏染料化合物。Fluorescein是熒光示蹤劑，可以應(yīng)用于醫(yī)學(xué)和生物學(xué)以及腫瘤感染的組織。體外研究Fluoresceinisnearlyinactiveasaphotodynamicsensitizer.Fluoresceinconsequentlyisalmostuselessasaphotodynamicsensitizer,butitisofgreatvalueinfluorescenceimmunoassays.Thefluoresceindyeisacommonfluorescentprobe.Itsveryhighmolarabsorptivityatthewavelengthoftheargonlaser(488nm),largefluorescencequantumyieldandhighphotostabilitymakesitaveryusefulandsensitivefluorescentlabel.Fluoresceiniscommerciallyavailableinmanyderivatives,suchasfluoresceinisothiocyanateandfluoresceinsuccinimidylester,thatcanbecovalentlyattachedtomacromoleculesandtoaminoacids.Thelabeledmoleculescanbedetectedwithveryhighsensitivitywhichisutilizedin,forexample,capillaryelectrophoresis.Theemissionspectrumoffluoresceinoverlapsextensivelytheabsorptionspectrumoftetramethylrhodamine,whichisarelatedstronglyfluorescentdye,makingthispairverysuitableforenergytransferexperimentstodeterminedistanceswithinandbetweenlabeledmacromolecules.Fluoresceininaqueoussolutionoccursincationic,neutral,anionicanddianionicformsmakingitsabsorptionandfluoresencepropertiesstronglypHdependent.化學(xué)性質(zhì)熒光素外觀呈橙黃或淺橙紅色結(jié)晶粉末。熔點(diǎn)314-316℃（分解）。溶于熱乙醇、冰醋酸、堿溶液，呈綠色螢光，不溶于水、苯、氯仿和乙醚。用途熒光素是汞溴紅的中間體，可用作螢光光度分析試劑，測(cè)定氯、溴、碘、硫離子；氧化-還原指示試劑；生物染色劑。用途熒光光度分析硫離子，試驗(yàn)溴，滴定氯、溴和碘。吸附指示劑，氧化還原指示劑。用途吸附指示劑；氧化還原指示劑；熒光光度分析硫離子；滴定氯、溴和碘生產(chǎn)方法熒光素可用鄰苯二甲酸酐與間苯二酚在硫酸參與下反應(yīng)制得。 |
| 結(jié)構(gòu)式： |
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金經(jīng)理 |
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